Chromatin Immunoprecipitation:Article Title: MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199
Article Snippet: .. Antibodies used for ChIP and ChIP-seq assays a-MLL-N (Bethyl, A300-086A, ChIP and ChIP-seq); a-AF4-C (Abcam, ab31812, ChIP, ChIP-seq and western blot); a-AF4-N (Bethyl, A302-344A, ChIP); a-ENL (Bethyl, A302-268A, ChIP and ChIP-seq); a-AF9 (Bethyl, A300-595A, ChIP); a-CDK9 (Bethyl, A303-493A, ChIP); a-CyclinT1 (Bethyl, A303-496A, ChIP); a-AFF4 (Bethyl, A302-538A, ChIP); a-MLL-C (Active Motif, 61295, ChIP); a-CFP1 (Bethyl A303-161A); a-DOT1L (Bethyl, A300953A, ChIP); a-H3K79me2 (Millipore 04-835, ChIP-seq; Active Motif 39143 ChIP) a-H3K79me3 (Diagenode pAb068-050, ChIP and ChIP-seq) ; a-H3 (Abcam, ab1791); a-H3K4me3 (Diagenode, C1541003 ChIP and ChIP-seq); aH3K27Ac (Diagenode, C15410196, ChIP and ChIP-seq); a-H3K4me1 (Diagenode, C15410194, ChIP-seq); a-CBX8 (Bethyl, A300-882A). .. The following Taqman primer-probe sets were purchased from Applied Biosytems (Waltham, MA, USA): b2M Hs99999007; GAPDH Hs03929097_g1; BCL-2 Hs00608023_m1; BAX Hs00180269_m1; MCL-1 Hs01050896_m1; BCL2L1 Hs00236329_m1; BCL2L11 Hs00708019; MLL Hs00172962_m1; ENL Hs00172962_m1; RUNX1 Hs00231079_m1.
Article Title: MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199
Article Snippet: .. Antibodies used for ChIP and ChIP-seq assays a-MLL-N (Bethyl, A300-086A, ChIP and ChIP-seq); a-AF4-C (Abcam, ab31812, ChIP, ChIP-seq and western blot); a-AF4-N (Bethyl, A302-344A, ChIP); a-ENL (Bethyl, A302-268A, ChIP and ChIP-seq); a-AF9 (Bethyl, A300-595A, ChIP); a-CDK9 (Bethyl, A303-493A, ChIP); a-CyclinT1 (Bethyl, A303-496A, ChIP); a-AFF4 (Bethyl, A302-538A, ChIP); a-MLL-C (Active Motif, 61295, ChIP); a-CFP1 (Bethyl A303-161A); a-DOT1L (Bethyl, A300953A, ChIP); a-H3K79me2 (Millipore 04-835, ChIP-seq; Active Motif 39143 ChIP) a-H3K79me3 (Diagenode pAb068-050, ChIP and ChIP-seq) ; a-H3 (Abcam, ab1791); a-H3K4me3 (Diagenode, C1541003 ChIP and ChIP-seq); aH3K27Ac (Diagenode, C15410196, ChIP and ChIP-seq); a-H3K4me1 (Diagenode, C15410194, ChIP-seq); a-CBX8 (Bethyl, A300-882A). .. The following Taqman primer-probe sets were purchased from Applied Biosytems (Waltham, MA, USA): b2M Hs99999007; GAPDH Hs03929097_g1; BCL-2 Hs00608023_m1; BAX Hs00180269_m1; MCL-1 Hs01050896_m1; BCL2L1 Hs00236329_m1; BCL2L11 Hs00708019; MLL Hs00172962_m1; ENL Hs00172962_m1; RUNX1 Hs00231079_m1.
Western Blot:Article Title: MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199
Article Snippet: .. Antibodies used for ChIP and ChIP-seq assays a-MLL-N (Bethyl, A300-086A, ChIP and ChIP-seq); a-AF4-C (Abcam, ab31812, ChIP, ChIP-seq and western blot); a-AF4-N (Bethyl, A302-344A, ChIP); a-ENL (Bethyl, A302-268A, ChIP and ChIP-seq); a-AF9 (Bethyl, A300-595A, ChIP); a-CDK9 (Bethyl, A303-493A, ChIP); a-CyclinT1 (Bethyl, A303-496A, ChIP); a-AFF4 (Bethyl, A302-538A, ChIP); a-MLL-C (Active Motif, 61295, ChIP); a-CFP1 (Bethyl A303-161A); a-DOT1L (Bethyl, A300953A, ChIP); a-H3K79me2 (Millipore 04-835, ChIP-seq; Active Motif 39143 ChIP) a-H3K79me3 (Diagenode pAb068-050, ChIP and ChIP-seq) ; a-H3 (Abcam, ab1791); a-H3K4me3 (Diagenode, C1541003 ChIP and ChIP-seq); aH3K27Ac (Diagenode, C15410196, ChIP and ChIP-seq); a-H3K4me1 (Diagenode, C15410194, ChIP-seq); a-CBX8 (Bethyl, A300-882A). .. The following Taqman primer-probe sets were purchased from Applied Biosytems (Waltham, MA, USA): b2M Hs99999007; GAPDH Hs03929097_g1; BCL-2 Hs00608023_m1; BAX Hs00180269_m1; MCL-1 Hs01050896_m1; BCL2L1 Hs00236329_m1; BCL2L11 Hs00708019; MLL Hs00172962_m1; ENL Hs00172962_m1; RUNX1 Hs00231079_m1.
Article Title: MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199
Article Snippet: .. Antibodies used for ChIP and ChIP-seq assays a-MLL-N (Bethyl, A300-086A, ChIP and ChIP-seq); a-AF4-C (Abcam, ab31812, ChIP, ChIP-seq and western blot); a-AF4-N (Bethyl, A302-344A, ChIP); a-ENL (Bethyl, A302-268A, ChIP and ChIP-seq); a-AF9 (Bethyl, A300-595A, ChIP); a-CDK9 (Bethyl, A303-493A, ChIP); a-CyclinT1 (Bethyl, A303-496A, ChIP); a-AFF4 (Bethyl, A302-538A, ChIP); a-MLL-C (Active Motif, 61295, ChIP); a-CFP1 (Bethyl A303-161A); a-DOT1L (Bethyl, A300953A, ChIP); a-H3K79me2 (Millipore 04-835, ChIP-seq; Active Motif 39143 ChIP) a-H3K79me3 (Diagenode pAb068-050, ChIP and ChIP-seq) ; a-H3 (Abcam, ab1791); a-H3K4me3 (Diagenode, C1541003 ChIP and ChIP-seq); aH3K27Ac (Diagenode, C15410196, ChIP and ChIP-seq); a-H3K4me1 (Diagenode, C15410194, ChIP-seq); a-CBX8 (Bethyl, A300-882A). .. The following Taqman primer-probe sets were purchased from Applied Biosytems (Waltham, MA, USA): b2M Hs99999007; GAPDH Hs03929097_g1; BCL-2 Hs00608023_m1; BAX Hs00180269_m1; MCL-1 Hs01050896_m1; BCL2L1 Hs00236329_m1; BCL2L11 Hs00708019; MLL Hs00172962_m1; ENL Hs00172962_m1; RUNX1 Hs00231079_m1.
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